Hypoxanthine Production from Nucleosides in Erythrocyte Ghosts
نویسندگان
چکیده
منابع مشابه
Hypoxanthine production from nucleosides in erythrocyte ghosts.
A primary event in the metabolism of nucleosides by human erythrocytes involves the action of a nucleoside phosphorylase (l-3). In the presence of arsenate, free ribose is produced from adenosine, inosine, guanosine, and xanthosine (4). Adenosine has been reported to be converted to adenine (4), but other studies have shown that adenosine was converted by deamination to inosine and then cleaved...
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Erythrocyte ghosts have been found to be peculiarly useful for the study of the metabolism of nucleosides (1, 2). Preparations made by osmotic hemolysis were found to be unable to metabolize glucose. Adenosine, however, caused a disappearance of inorganic phosphate from ghost plasma suspensions with an equivalent production of organic acid-soluble ester phosphate. There was a simultaneous disap...
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1. Erythrocyte ghosts from human blood were produced by gentle water hemolysis. The ghost-containing hemolysate (about 20 mN) was added to media of different composition (KCl, NaCl, glucose, sucrose, etc.) and varying concentration ranging from 8 to 840 mN. The volume changes of the ghost cells were followed by a light absorption method. The potassium and sodium concentrations were also analyze...
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The underlying mechanism of the low-frequency dielectric dispersion, called the alpha dispersion, of osmotically lysed erythrocytes (erythrocyte ghosts) has remained open since its finding [H. P. Schwan and E. L. Carstensen, Science 125, 985 (1957)]. The alpha dispersion is peculiar to erythrocyte ghosts and has never been observed for intact erythrocytes. Numerical calculation based on interfa...
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We have used freeze-etching and SDS-polyacrylamide gel electrophoresis to study the conditions under which the intramembrane particles of the human erythrocyte ghost may be aggregated. The fibrous membrane protein, spectrin, can be almost entirely removed from erythrocyte ghosts with little or no change in the distribution of the particles. However, after spectrin depletion, particle aggregatio...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1959
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)69658-9